Identification of Small Molecules that Disrupt Signaling between ABL and Its Positive Regulator RIN1
Fig 3
Representative data from primary HTS.
Example of 384-well plate data presented as a scatterplot. Each plate was screened with positive (purple) and negative (blue) controls consisting of streptavidin terbium and ABL-eGFP with and without RIN1-SBP, respectively. Screening compounds are represented in gray. The hit cutoff was a plate-based 3-standard deviation decrease in the FRET ratio.