The In Vitro and In Vivo Anti-Cancer Activities of a Standardized Quassinoids Composition from Eurycoma longifolia on LNCaP Human Prostate Cancer Cells
Fig 6
Protein expression of G1/S regulatory proteins in LNCaP cells treated with SQ40.
LNCaP cells were first cultured in growth media supplemented with 5% CSS for 48 hours and then treated with 3, 6 and 12 μg/mL of SQ40 with the presence of 100 nM DHT for 72 hours. Immunoblotting was performed on protein extracts to detect CDK4, CDK2, Cyclin D1, Cyclin D3, p21Waf1/Cip1 and p27Kip1. β-actin served as a loading control.