Regulation of Lipid Droplet Size in Mammary Epithelial Cells by Remodeling of Membrane Lipid Composition—A Potential Mechanism
Fig 6
Different size distribution of lipid droplets in the medium induced by specific free fatty acids (FFAs).
After cultivating mammary epithelial cells with 100 μM FFA (palmitic or oleic acid) or with FFA-free medium (control) for 24 h, medium was collected and lipid droplets were stained with Nile red. Representative images of lipid droplets in the medium collected from palmitate and oleate treatments (A and B, respectively). Droplets were measured and divided into three size groups: 0 > X < 3, 3 > X < 5, and ≤5 μm. Size distribution of lipid droplets in the medium was compared by chi-square test (C). Scale bar = 20 μm.