Regulation of Lipid Droplet Size in Mammary Epithelial Cells by Remodeling of Membrane Lipid Composition—A Potential Mechanism
Fig 3
Transcription levels of activity markers of mitochondria and phospholipid converting enzyme are modulated by free fatty acids (FFAs).
Mammary epithelial cells were treated with 100 μM FFA (capric, palmitic or oleic acid) or with FFA-free medium (control) for 2 h; RNA was extracted and gene-expression levels of (A) PGC-1α, (B) PGC-1β, (C) NDUFAF3 and (D) PEMT were analyzed by real-time PCR. All data are presented as mean ± SEM of the expression level of the assayed gene normalized to the geometric mean of two housekeeping genes. Different letters indicate significant differences between treatment groups (P < 0.05).