Hepatocyte Nuclear Factor 1A (HNF1A) as a Possible Tumor Suppressor in Pancreatic Cancer
Fig 5
Effect of HNF1A knockdown on apoptosis.
72 h after transfection with anti-HNF1A siRNAs, the AsPC-1 and BxPC-3 cells were stained with FITC conjugated Annexin-V/PI. Percentage of Annexin-V/PI-stained cells was determined by flow cytometry. Dot plot was divided into a quadrant: (1) necrotic cells, (2) late apoptotic cells, (3) living cells, and (4) early apoptotic cells. Bar graph shows the percentage of apoptotic cells. Values are expressed as mean ± SD from three different experiments. *P<0.05 compared with the control.