Derricin and Derricidin Inhibit Wnt/β-Catenin Signaling and Suppress Colon Cancer Cell Growth In Vitro
Fig 7
Derricin and derricidin inhibit double axis formation in Xenopus embryos and suppress Wnt/β-catenin luciferase reporter activity in vivo.
Representative images of embryos co-injected with xWnt8 mRNA plus DMSO (B) or xWnt8 mRNA plus derricin or derricidin (C,D). Observe the incomplete double axis formation or secondary axis ablation in embryos injected with chalcones. (E-F) Graphs show percentages of embryo phenotypes. Observe that co-injection of chalcones with xWnt8 mRNA reduced the percentage of embryos with double axis, and increased the proportion of normal embryos, with one axis. (G) Xenopus embryos injected with S01234 reporter + Renilla, xwnt8 (10pg) with DMSO or the chalcones (0.4 pmol/embryo). The activation of the reporter was prevented by chalcone injection. Arrowheads: cement gland; arrow: incomplete double axis; A-P: anterior-posterior axis.