Derricin and Derricidin Inhibit Wnt/β-Catenin Signaling and Suppress Colon Cancer Cell Growth In Vitro
Fig 4
Derricin and derricidin induced cell cycle arrest in CRC cells.
(A, D, E) In HCT116, untreated cells showed approximately 75% of cells in G1/G0 phase, while 30 and 50 μM of derricin reduced this proportion to 67.6% and 54%, respectively, resulting in cell cycle arrest in S and G2/M phases, respectively. Derricidin reduced this proportion to 53.8% and 35.5%, respectively and also induced cell cycle arrest in G2/M phase. (B, F,G) On the other hand, DLD-1 cells accumulated in G0/G1 phase of cell cycle after 30 μM and 50 μM of derricin or derricidin. (C, H, I) Derricin and derricidin treatment had milder effects in cell cycle progression of IEC-18 cells, except for a slightly arrest at G2/M with 50 μM of derricin. Bar graphs represent the percentage of cells according treatment and significant data were represented in the figure. Black bars: G0/G1 phase; White bars: S phase; and Gray bars: G2/M phases.