Derricin and Derricidin Inhibit Wnt/β-Catenin Signaling and Suppress Colon Cancer Cell Growth In Vitro
Fig 2
Derricin and derricidin reduce cell number and cell viability of colon cells.
(A, B, D, E, G, H) Graphs show MTT absorbance levels in HCT116, DLD-1 and IEC-18 cell lines, after treatment up to 72 h with 10–100 μM of derricin or derricidin. (A) In HCT116 cells, it is observed significant reduction in cell viability after 100 μM of derricin for 24 h and 20 μM of derricin for 48 h (B) After derricidin treatment, reduction of cell viability occurred at 100 μM of derricidin after 24 h and at 30 μM after 72 h. (D) In DLD-1 cells, it is observed a milder effect at 100 μM after 18h of treatment. Otherwise, an intense reduction of MTT absorbance is detected after 48h treatment with 100 μM of derricin. (E) Derricidin treatment significantly reduces DLD-1 cell viability after 18h treatment with 50 μM of flavonoid and at 20 μM after 72h. (G) In IEC-18 cells, reduction of viability is observed with 100 μM of derricin, starting at 18h of treatment. (H) A similar profile is observed after derricidin treatment. (C,F,I) DAPI-stained nuclei were counted after 24h treatment with derricin and derricidin. (C) 30 and 50μM of derricin reduced approximately 30% and 58% the number of HCT116 nuclei, respectively; while derricidin reduced approximately 39% and 65%, respectively. (F) DLD-1 nuclei were decreased about 44% and 50% after derricin treatment at 30 and 50 μM concentrations, respectively. Derricidin treatment diminished about 50% and 70%, respectively. (I) IEC-18 nuclei were also decreased, with values of 25% and 32% of reduction with 30 and 50μM of derricin, respectively, and 40% and 38% with 30 and 50μM of derricidin, respectively. * p<0.05, **p<0.01, ***p<0.001.