DNA Methyltransferase Inhibitor Zebularine Induces Human Cholangiocarcinoma Cell Death through Alteration of DNA Methylation Status
Fig 5
Effects of zebularine on apoptotic cell death in HuCCT1 cells.
HuCCT1 cells were treated with zebularine at indicated concentrations for 72 h. Necrosis (A) and apoptosis (B) were measured using apoptotic/necrotic/healthy cell detection assay. (C) Apoptosis also was measured by Cell Death Detection ELISA assay (C). The data are expressed as fold-increases relative to the respective untreated samples. Caspase-3/7 (D), -8 (E), and -9 (F) activities were determined using Caspase-Glo Assays. The data are expressed as fold-increases relative to the respective untreated samples (RLU/60 min/mg protein). (G) Forward transfection of negative control siRNA (NC) as the control, siDNMT1#1 or siDNMT1#2 at 30 nM was performed, and apoptosis was measured by Cell Death Detection ELISA assay. Data are the means ± SEM of results from at least three independent experiments. *p < 0.05, compared to 0 μM.