Optomotor-Blind Negatively Regulates Drosophila Eye Development by Blocking Jak/STAT Signaling
Fig 3
Ectopic omb expression can block morphogenetic furrow initiation, progression, and differentiation.
Flip-out induced omb expression clones (Act5C>omb) marked by GFP coexpression repressed Elav (cyan) and Eya (red) expression. (A-A”) A clone at the posterior margin (arrow) inhibited MF initiation. (B-B”) A clone at the MF (arrow) inhibited MF progression (as indicated by CycB pattern, red) and neuronal differentiation (Elav, cyan). (C-C”) Omb expression level in Act5C>omb clones varied. Omb expression in a single ommatidial clusters (arrows) could autonomously block neuronal differentiation (Elav, cyan). The Z-section along the white line is shown in C-1 to C-1”. The relative level of Omb induction correlates to the signal of coexpressed GFP. (D) Tangential semi-thin sections through an adult eye of an hs-omb transgenic fly exposed to a single 1hr 37°C heat shock during mid-L3. Ommaditial patterning resumed normally beyond the dorso-ventral scar (arrow).