Optomotor-Blind Negatively Regulates Drosophila Eye Development by Blocking Jak/STAT Signaling
Fig 1
omb expression level influences eye size.
(A) w ombP1 (an enhancer trap insertion that does not affect omb expression and function, Sun et al., 1995), (B) w ombP1 l(1)omb3198/w ombbi. The expanded territory of ventral eye fate is clearly evident. Because of the increased size, the eye surface is more convex. Therefore, the unaffected dorsal pigmentation is not fully visible under this angle. (C) ombP3>omb-RNAi showed strong overgrowth in the eye. The overgrowth is stronger in the ventral than in the dorsal part of the eye. The eye is convoluted. (D-E) mirr-lacZ (anti-beta-galactosidase, red). Phalloidin staining (green). Elav (blue). (D-D”’) mirr-lacZ/+ eye disc showing the dorsal-specific expression of mirr-lacZ. D, D’ and D”, D”’ are two focal planes. The D”, D”’ focal plane shows the ventral flap. (E-E”’) l(1) ombD4/ ombP7 eye disc. E, E’ and E”, E”’ are two focal planes. The E”, E”’ focal plane shows the ventral flap. The dorsal and ventral eye regions were distinguished (separated by a white line) based on mirr-lacZ and the position of the optic stalk. Two different focal planes are acquired in each eye disc. The area of eye disc including the ventral flap, based on two focal planes, were measured by the software, Zeiss Zen 2009. The results are summarized in (F). The ventral area of l(1)ombD4/ombP7; mirr-lacZ/+ are significantly enlarged compared to that of mirr-lacZ/+. The dorsal area of l(1)ombD4/ombP7; mirr-lacZ/+ are not significant increased compared to that of mirr-lacZ/+. Differences (*) presented in (E) and (J) are significant (Student′s t-test, **, p<0.05; n.s., non-significant). In all panels anterior is left and dorsal up.