Nfix Expression Critically Modulates Early B Lymphopoiesis and Myelopoiesis
Fig 6
Loss of Nfix perturbs myelopoiesis while driving B cell differentiation.
Total BM from WT or Nfix deficient mice was plated onto OP9 cells and analysed by flow cytometry on day 4 (A) and day 6 (B). Bar chart shows the mean percentages of B220+ cells from 3 independent experiments +/- SD. (C) Representative flow cytometric analysis of cells expressing B220 at day 6. (D) Total BM from WT or Nfix deficient mice was plated in methylcellulose (M3434). Colonies were counted after 11 days. Bar chart represents the mean number of colonies from 2 independent experiments +/- SD. (E) Bar chart shows the mean percentages of Gr1 + and F4/80+ cells from 2 independent experiments +/- SD. (F) Representative flow cytometric analysis of cells from colony assay which were stained with Gr-1 and F4/80. (G) Graphical presentation of relative mRNA expression of indicated genes assessed using high-throughput qPCR on the 48.48 Dynamic Array IFC system (Fluidigm). Bars represent the average of 3 biological Nfix-/- replicates normalized to WT control, and error bars denote +/- SD.