Nfix Expression Critically Modulates Early B Lymphopoiesis and Myelopoiesis
Fig 2
Decreased B cells and increased myeloid cells in Nfix expressing cells in the bone marrow and spleen.
C57Bl/6 mice were reconstituted with BM cells transduced with control MigR1 or Nfix vectors. Graph of percentage of B cells (left, B220+CD19+) and myeloid cells (right, Gr-1+CD11b+) in the (A) BM and (B) Spleen of MigR1 and Nfix chimeric animals (GFP+) 10 wks post-transplant. Representative flow cytometric analysis in MigR1 and NFIX chimeric animals, 10 wk post transplant, showing GFP engraftment (left panel), B220+CD19+ B cells (middle panel, percentages given) and Gr-1+CD11b+ myeloid cells (right panel) in the GFP- and GFP+ fractions of the bone marrow (C) and spleen (D). Results are representative of 2 independent BMT experiments.