2H,3H-Decafluoropentane-Based Nanodroplets: New Perspectives for Oxygen Delivery to Hypoxic Cutaneous Tissues
Fig 3
OLND internalization by HaCaT cell line.
Human keratinocytes (106 cells /2 ml Panserin medium) were left untreated (upper panels) or treated with 200 l FITC-conjugated OLND PBS formulation (lower panels) for 24 h in normoxia (20% O2). After DAPI staining, cells were checked by confocal microscopy. Results are shown as representative images from three independent experiments. Left panels: cell nuclei after DAPI staining, in blue. Central panels: FITC-conjugated OLNDs, in green). Right panels: merged images. Magnification: 63X.