Dynamics of Sun5 Localization during Spermatogenesis in Wild Type and Dpy19l2 Knock-Out Mice Indicates That Sun5 Is Not Involved in Acrosome Attachment to the Nuclear Envelope
Fig 5
Sun5 is removed during spermiogenesis along an antero-caudal axis.
(A) In early condensing spermatids, Sun5 is located at the base of the nucleus. Elongating spermatids co-stained with anti-Sp56 antibodies (A1, red) and anti-Sun5 Ab1 (A2, green) and counterstained with Hoechst to evidence the nucleus (blue). Arrow heads show specific Sun5 staining at the implantation fossa in late condensing spermatids. (B) In early condensing spermatids, Sun5 is no longer present in Golgi apparatus. Elongating spermatids co-stained with anti-GM130 antibodies (B1, purple) and anti-Sun5 Ab1 (B2, green) and counterstained with Hoechst to evidence the nucleus (blue). (C) In round spermatids (white arrows), Lamin B1 (purple) is not located in front of the acrosomal vesicle (evidenced by anti-Sp56 antibodies green), showing that lamin B1 and Sun5 were both excluded from the acrosomal area. In early condensing spermatids (yellow arrow heads), Lamin B1 (purple) is absent. Cells were counterstained with Hoechst to evidence the nucleus (blue).