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Potential Contribution of Phenotypically Modulated Smooth Muscle Cells and Related Inflammation in the Development of Experimental Obstructive Pulmonary Vasculopathy in Rats

Fig 2

Cellular components in intimal and plexiform lesions.

Photomicrographs (elastic van Gieson staining, EVG) of sprouting intimal lesion (Panels Aab) and sprouting plexiform lesion (Panel Ac) in rats 3 weeks after initial treatment. Serial sections of longitudinal views (Panels Ba-h) and cross sectional views (Panels Bi-l) of intimal lesions and serial sections of plexiform lesions (Panels Bm-p) in EVG, von Willebrand factor (VWF), α-smooth muscle actin staining (αSMA) and negative controls. Photomicrographs (confocal microscopy) of a section of the intimal and plexiform lesion (Panels C), by using antibodies for VWF and αSMA, were presented. TO-PRO-3, nuclear staining. Arrows indicate abnormal cells.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0118655.g002