Monoclonal Antibodies Directed to Fucoidan Preparations from Brown Algae
Fig 2
Sulfate assay and de-sulfation.
(A) Azure A absorbance spectra changes in the presence of differing levels (0 to 50 μg) of sulfated dextran, dextran and FS28. (B) Effect of de-sulfation on BAM MAb recognition of FS28. Samples of FS28 were collected over 4 time points (0, 1, 2 and 3 h) during a de-sulfation treatment with chlorotrimethylsilane and assayed using ELISA. Absorbance values are means of 4 replicates and error bars indicate SD. Sulfate levels as equivalents to native FS28 (w/w).