Defective Differentiation of Adipose Precursor Cells from Lipodystrophic Mice Lacking Perilipin 1
Fig 7
Enhanced induction improves Plin1-/- SVC differentiation.
Epididymal SVCs were induced for 2 days (D1~2) in differentiation medium (IDMI) containing 250 μM IBMX, 17 nM insulin, 0.1 μM dexamethasone and 60 μM indomethacin, followed by an additional 2-day (D3~4) treatment with 17 nM insulin. Lipid droplets were stained with Oil-red O. (A) Effect of IBMX dose on lipid-droplet formation and adipocyte differentiation of Plin1+/+ and Plin1-/- SVCs. (B) Effect of replacing indomethacin with 5 μM rosiglitazone (IDMR) on lipid-droplet growth and adipogenesis in SVCs. (C) After day-4 differentiation, SVCs were further stimulated with 17 nM insulin (Ins), 400 μM oleic acid (OA), or both for an additional 2 days (D5~6).