Defective Differentiation of Adipose Precursor Cells from Lipodystrophic Mice Lacking Perilipin 1
Fig 3
Flow cytometric analysis of SVC cells.
The stromal vascular cells (SVCs) were isolated from epididymal fat of Plin1+/+ and Plin1-/- mice and underwent analysis. Adipocyte progenitors in the SVC fraction were immunostained simultaneously with antibodies to CD34 (Alexa Fluor 647), CD29 (FITC), Sca-1 (PE), and CD24 (PerCP-Cy5.5). Cells were incubated with fluorescent isotype-matched IgG as negative controls and single-color positive cells were stained with each antibody alone. The CD29+:CD34+ adipocyte progenitor population was gated (in box) and further separated for the subpopulation coexpressing Sca-1 and CD24 (CD29+:CD34+:Sca-1+:CD24+). The percentage of adipocyte progenitor population in SVC cells was denoted in box.