Defective Differentiation of Adipose Precursor Cells from Lipodystrophic Mice Lacking Perilipin 1
Fig 1
Aberrant morphology of adipose tissue in Plin1-/- mice.
(A) Hematoxylin and eosin (HE) staining of adipose tissues in Plin1-/- and Plin1+/+ male mice at 8 and 25 weeks old. The boxed fields (100×) were showed underneath at high magnification (400×). (B) Cumulative adipocyte frequency from epididymal adipose tissues of Plin1-/- and Plin1+/+ mice (n = 3 for each genotype) at age of 25 weeks. For each mice, 10~12 fields of vision at 100 × magnification from different segments of fat tissue were randomly selected for analysis. A total 6,265 of Plin1+/+ unilocular adipocytes (circles) and 11,471 Plin1-/- unilocular (squares) and 1,881 Plin1-/- multilocular (triangles) adipocytes were counted. Cell area was measured by use of NIH Image-J software. The y-axis values represent the cumulative cell percentage for adipocytes at and below the corresponding sizes on the x-axis. The lines labeled as 50th percentile intersect the curves at the median cell sizes (boxes) below which 50% of the adipocytes in each population were distributed. The leftward shift of the curves indicates that the adipocyte population in Plin1-/- mice tend toward smaller cell area as compared with that in Plin1+/+ mice. The inset panel shows the relative (non-cumulative) cell frequency versus cell area, and demonstrates that Plin1-/- mice have a greater proportion of small adipocytes than Plin1+/+ mice.