A Rapid Optical Clearing Protocol Using 2,2′-Thiodiethanol for Microscopic Observation of Fixed Mouse Brain
Figure 1
Fixed brain tissue clearing with TDE solutions.
(a, b) Fixed adult mouse brains after treatment with different concentrations of TDE solutions. Whole brains (a) and brain slices (400 µm in thickness) (b) were immersed in each TDE solution for 2 days and 1 h, respectively. The photograms were taken under backlighting. (c) Transmission curves of fixed brain slices (400 µm in thickness, n = 3) after 2 h of immersion in each TDE solution. Data shown represent the average ± SEM.