Enzymes from Fungal and Plant Origin Required for Chemical Diversification of Insecticidal Loline Alkaloids in Grass-Epichloë Symbiota
Figure 6
Assay of LolM methyltransferase activity.
(A) Chromatogram of loline alkaloids from incubation of norloline and AdoMet with protein extract of yeast transformed with empty vector. (B) Chromatogram of loline alkaloids from incubation of norloline and AdoMet with crude protein extract from yeast that expresses LolM. (C) Chromatogram of loline alkaloids from incubation of loline and AdoMet with protein extract of yeast transformed with empty vector. (D) Chromatogram of loline alkaloids from incubation of loline and AdoMet with crude protein extract from yeast that expresses LolM. (E) Proposed scheme of loline and N-methylloline (NML) formation from norloline. AdoHcy = S-adenosyl homocysteine.