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Enzymes from Fungal and Plant Origin Required for Chemical Diversification of Insecticidal Loline Alkaloids in Grass-Epichloë Symbiota

Figure 2

Enrichment of deuterated N-acetylnorloline (NANL) from application of tetradeuterated exo-1-acetemidopyrrolizidine ([2H4]AcAP) to loline alkaloid producing culture.

Shown are (A) GC-MS total ion chromatogram, (B) mass spectrum at retention time 13.948 and (C) 13.921 min, and (D) proposed scheme of [2H4]NANL formation from [2H4]AcAP.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0115590.g002