Abieslactone Induces Cell Cycle Arrest and Apoptosis in Human Hepatocellular Carcinomas through the Mitochondrial Pathway and the Generation of Reactive Oxygen Species
Figure 4
The effect of abieslactone on the cell cycle and the expression of cell cycle regulators in HepG2 and SMMC7721 cells.
(A) Abieslactone treatment induced a dose dependent increase in the proportion of cells in the G1 phase and a decrease in cells in the S and G2 phases compared to the untreated control. Z-VAD-FMK treatment did not prevent cell cycle arrest following high-dose abieslactone treatment. The results are represented as the mean ± SEM for three independent experiments with similar results. *P<0.05; **P<0.01 vs. the untreated control. (B) Representative pictures for p53, p21 CDK2 and cyclin D1 protein expression by western blot analysis. β-actin was used as a control.