Inhibitory Activity of the Isoflavone Biochanin A on Intracellular Bacteria of Genus Chlamydia and Initial Development of a Buccal Formulation
Figure 3
Impact of biochanin A on C. pneumoniae replication.
A) Inhibition of C. pneumoniae inclusion counts and infectious progeny production by different concentrations of biochanin A. B) Effect of host cell or elementary body (EB) pretreatment with biochanin A on C. pneumoniae inclusion counts. C) Effect of delayed administration of biochanin A on the inhibitory capacity of biochanin A on C. pneumoniae inclusion counts (1st passage) and infectious progeny production (2nd passage). Biochanin A (50 µM) was added into infected cell cultures at 2, 6, 12 or 24 h post infection. In all experiments, the cultures were stained at 72 h as described in Materials and Methods. *** In B) and C) indicates statistically significant difference (p<0.05; unpaired t-test) compared to untreated control infection.