Cellular Responses during Morphological Transformation in Azospirillum brasilense and Its flcA Knockout Mutant
Figure 5
Differential gene expression in Sp7 and Sp7-flcAΔ under both nutrient and flocculation conditions, analyzed by qRT-PCR.
Transcription levels of atpD, clpP, glnA, livK, nark, narL1, nos and phaA were significantly higher in Sp7-flcAΔ than in Sp7 under flocculation conditions. Transcription levels of cheW, galU and gltA were significantly lower in Sp7-flcAΔ than in Sp7 under flocculation conditions. Transcription levels of ahpC, gloA, lpxC and sodA did not differ significantly in Sp7 and Sp7-flcAΔ under either culture condition. * indicates a significant difference between group means (P<0.05). Data is presented as mean ±SEM, relative to expression of two reference genes, n = 4 biological replicates.