A Chemical Proteomics Approach for the Search of Pharmacological Targets of the Antimalarial Clinical Candidate Albitiazolium in Plasmodium falciparum Using Photocrosslinking and Click Chemistry
Figure 5
Protein interaction networks were built in Cytoscape 2.5.1 using the PlasmoID plugin. Three clusters of UA1936-interacting proteins were found based on the following biological process terms: “phospholipid metabolism” depicted in pink, “vesicle-mediated transport” in blue and “transport” in violet. A fourth cluster of unknown or unrelated functions was created and named “miscellaneous”, as depicted in orange. For each protein (node), the PlasmoDB ID accession number is indicated in a colored rectangle followed by the putative name of the gene. Proteins in dark-colored rectangles were found in the three replicated experiments and proteins in light-colored rectangles were found in two replicates. The proteins depicted in ellipses are interacting proteins identified in the PlamsoMap interactome analysis with their respective likelihood scores. The colors on the edges in the network represent: Codes for (black), Interacts with (blue), and Participates in (orange). The p values for the gene ontology terms found to be significantly enriched are presented for genes having those annotations in the biological process.