Bacterial Fucose-Rich Polysaccharide Stabilizes MAPK-Mediated Nrf2/Keap1 Signaling by Directly Scavenging Reactive Oxygen Species during Hydrogen Peroxide-Induced Apoptosis of Human Lung Fibroblast Cells
Figure 5
The protective effect of HFC polysaccharide on H2O2-induced DNA damage during cell cycle progression.
WI38 cells were incubated in presence (100, 200 and 250 µg/mL) or in absence of HFC polysaccharide for 1 h followed by the treatment with 300 µM H2O2 in both the cases for varying periods of time (0–24 h). The cell cycle progression was assayed by PI staining with flow-cytometry. The corresponding data are shown as bar graphs. Results are representative of three independent experiments performed in triplicate and are represented as mean ± SD. A one-way analysis of variance (ANOVA, Bonferroni corrections for multiple comparisons) was performed, where significant level stands for *p<0.05, **p<0.001, ***p<0.0001.