Synthesis and Functional Characterization of Substituted Isoquinolinones as MT2-Selective Melatoninergic Ligands
Figure 4
Stimulation of intracellular Ca2+ mobilization in CHO cells expressing MT1 or MT2 by isoquinolinone derivatives.
CHO cells expressing MT1 or MT2 were subjected to the treatment of increasing doses of selected tested compounds. Data were mean of peak fluorescence signals ± SEM of at least 3 different trials performed in triplicates, and normalized to the maximal response elicited by melatonin (as 100%) and the minimal response of vehicle-treated cells (as 0%). Estimation of maximal responses and EC50 were tabulated in Table 2.