Improving the Expression of Recombinant Proteins in E. coli BL21 (DE3) under Acetate Stress: An Alkaline pH Shift Approach
Figure 5
Effect of alkaline pHs on recombinant protein expression by E. coli BL21 (DE3).
A) GST, B) CYP and C) GFP. The protein expression was conducted at pH 6.5, 7.5 and 8.5 in 20 mL of BYT-glycerol medium in 250-mL shake flasks as described in Methods and Materials.