TCR Triggering by pMHC Ligands Tethered on Surfaces via Poly(Ethylene Glycol) Depends on Polymer Length
Figure 1
Schematic illustration of IEkMCC ligands tethered onto a plastic surface with PEG polymer linkers.
IEkMCC proteins with free c-terminal cysteines were first conjugated with heterobifunctional PEG linkers Mal-PEG-Bio through interactions between the sulfhydryl group and the maleimide group. Conjugates with biotin at the free ends of the polymer were then tethered to a plastic surface coated with streptavidin.