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The Role of Water Channel Proteins in Facilitating Recovery of Leaf Hydraulic Conductance from Water Stress in Populus trichocarpa

Figure 5

Relative expression of aquaporin genes in detached leaves during a dehydration-rehydration experiment.

Data are from control leaves (C) after they were perfused with artificial xylem sap (AXS) for 2 h, leaves that were dehydrated on the bench top for 1 h (D), and leaves that were dehydrated on the bench top for 1 h and then perfused for 2 h with AXS (RW). The geometric mean of the expression levels of four reference genes (ACT2, CYC063, TIP41-like, UBQ7) was used to normalize the results. Asterisks denote significant differences in expression level compared to control levels (one-way ANOVA, followed by Bonferroni’s post test, *P≤0.05; **P≤0.01***P≤0.001). Data are means ± SE of three biological replicates.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0111751.g005