Methyllycaconitine Alleviates Amyloid-β Peptides-Induced Cytotoxicity in SH-SY5Y Cells
Figure 5
MLA inhibits Aβ-induced autophagy process in SH-SY5Y cells.
(A) Western blot analysis of the protein level of LC3 in SH-SY5Y cells treated with 10 µM Aβ25–35 with or without MLA. (B) Immunofluorescence microscopy of punctate pattern of LC3 localization in SH-SY5Y cells treated with 10 µM Aβ25–35 with or without MLA. (C) Fluorescence microscopy of the formation of acidic vesicles with MDC staining in SH-SY5Y cells treated with 10 µM Aβ25–35 with or without MLA. (D) Flow cytometry of the formation of acidic vesicles after MDC staining in SH-SY5Y cells treated with 10 µM Aβ25–35 with or without MLA.