A Polyketide Synthase Acyltransferase Domain Structure Suggests a Recognition Mechanism for Its Hydroxymalonyl-Acyl Carrier Protein Substrate
Figure 5
Transacylase assay of ZmaA-AT Distinguishes ACP from Acyl Unit Recognition.
SDS-PAGE of reaction mixtures and corresponding phosphorimage. Lane 1: Molecular mass markers (Prestained Broad-range, Biorad). Lane 2: ZmaA-AT. Lane 3: ZmaD (ACP). Lane 4: Sfp (4′-phosphopantetheinyl transferase). Lane 5: ZmaA-AT, Sfp, and *Malonyl-CoA. Lane 6: ZmaA-AT, Sfp, ZmaD, and *Malonyl-CoA. Lane 7: ZmaA-AT, Sfp, and *(2-RS)-methylmalonyl-CoA. Lane 8: ZmaA-AT, Sfp, ZmaD, and *(2-RS)-methylmalonyl-CoA.