Genetic Diversity of the Coat Protein of Olive Mild Mosaic Virus (OMMV) and Tobacco Necrosis Virus D (TNV-D) Isolates and Its Structural Implications
Figure 2
Phylogenetic tree analysis of OMMV and TNV-D isolates based on CP nt sequences.
NJ tree was constructed from the sequence alignment of ca. 870 nts of OMMV and TNV-D CP coding region from 65 clones of OMMV and 55 TNV-D isolates and from 11 sequences retrieved from the GenBank database, totalling 131 sequences. Repeated sequences within each isolate were omitted. Each sequence variant was named with a letter (A, B, C,…) following the isolate designation to allow differentiation. Phylogenetic analysis included 78 sequences. Multiple sequence alignments were generated using MEGA 5.1, and phylogenetic tree was constructed by the NJ algorithm, based on calculations from pairwise nt sequence distances for gene nt analysis. Bootstrap analysis was done with 1000 replicates. Numbers above the lines indicate bootstrap scores out of 1000 replicates.