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The Cytochrome P450 Epoxygenase Pathway Regulates the Hepatic Inflammatory Response in Fatty Liver Disease

Figure 3

Atherogenic diet administration suppresses the CYP epoxygenase pathway.

(A) Plasma sum EET levels and (B) liver sum EET levels were significantly lower in mice administered the atherogenic diet compared to mice administered the STD diet (n = 4–6 per group). (C) The EET formation rate in the presence of saturating arachidonic acid concentrations was also suppressed in liver microsomes isolated from mice administered the atherogenic diet compared to mice administered the STD chow diet (n = 8 per group). (D) Liver Cyp2c29, Cyp2c50, Cyp2c55, and Cyp2j5 mRNA levels were significantly suppressed but (E) Ephx2 mRNA levels were not significantly different (STD diet: n = 6, atherogenic diet n = 5). *P<0.05 vs. STD diet group.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0110162.g003