Nucleolin Down-Regulation Is Involved in ADP-Induced Cell Cycle Arrest in S Phase and Cell Apoptosis in Vascular Endothelial Cells
Figure 3
Nucleolin protein down-regulation is involved in ADP-induced cell cycle arrest in S phase.
(A) ADP induced cell cycle arrest in S phase. HUVEC were treated with the indicated concentrations of ADP for 72 h. Medium was replaced with fresh medium containing ADP every day. The detached cells were removed, and the adhered cells were collected for cell cycle test by PI staining. (B) Quantitation of cell cycle arrest in S pahse induced by ADP in (A). * P<0.05 compared with the control group. (C) ADP induced time-dependent cell arrest in S phase in HUVEC. * P<0.05 compared with the control group. (D) Nucleolin over-expression partly reversed ADP-induced cell cycle arrest in S phase. Nucleolin-over-expressed HUVEC were treated with the indicated concentrations of ADP for 72 h. Medium was replaced with fresh medium containing ADP every day. The cell cycles were measured by PI staining. Mock-transfected HUVEC were used as controls. * P<0.05 compared with the control groups.