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Site-Specific Chemoenzymatic Labeling of Aerolysin Enables the Identification of New Aerolysin Receptors

Figure 5

Aerolysin imaging.

Aerolysin variants, fluorescently labeled, bind to the cell surface of HeLa cells. Images were acquired by confocal fluorescence microscopy. A Single labeled aerolysin versions. For comparable signal intensity, different aerolysin concentrations were required as indicated. B Double-labeled aerolysin and unlabeled aerolysin control.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0109883.g005