Cell Cycle Regulation of DNA Polymerase Beta in Rotenone-Based Parkinson's Disease Models
Figure 5
The cell cycle distribution in>4N phase and endoreduplication were interrupted by treatment with a DNA poly β inhibitor or by DNA poly β knockdown.
(A) The expression of DNA poly β was examined by western blot as cells cultured with DDC or were transfected with siRNA DNA poly β. The results are presented as the ratio of the DNA poly β expression to β-actin expression. * P<0.05 compared to the control group; ** P<0.05 compared to the rotenone group; Rot: rotenone. (B) and (C) Cell cycle distribution and DNA replication were analyzed by flow cytometry using BrdU/PI staining after the addition of DDC or DNA poly β depletion. * P<0.05 compared to the rotenone group; # P>0.05 compared to the rotenone group; R3 represents endoreduplication; R2-R3 represents S phase; The data represent three independent experiments and are expressed as the mean ± SD.