Hepatic Carboxylesterase 1 Is Induced by Glucose and Regulates Postprandial Glucose Levels
Figure 5
CES1 regulates postprandial levels.
(A–H) C57BL/6 mice were injected with Ad-shLacZ or Ad-shCes1. After 5 days, mice were fasted for 16 h followed by gavage with saline or glucose (8 g/kg) (n = 6 mice per group). Blood glucose levels were measured 1 h after gavage using a glucometer (A). Mice were then sacrificed 3 hours after gavage. Hepatic triglyceride (TG) (B) and free fatty acid (FFA) (C) levels were analyzed. Hepatic protein levels were assessed by Western blot assays (D) and then the ratio of p-AKT to total AKT was quantified (E). Hepatic mRNA levels of Ces1 (F), PEPCK (G) and G6Pase (H) were determined by qRT-PCR. (I) Reciprocal regulation between plasma glucose and hepatic CES1. Elevated plasma glucose induces hepatic CES1, which in turn helps lower plasma glucose levels likely via increasing peripheral insulin sensitivity. AKT, protein kinase B. *p<0.05 **p<0.01.