Neonatal NMDA Receptor Blockade Disrupts Spike Timing and Glutamatergic Synapses in Fast Spiking Interneurons in a NMDA Receptor Hypofunction Model of Schizophrenia
Figure 2
Postnatal NMDA receptor blockade destabilizes spike threshold in neocortical layer IV FSIs.
(A, B) Representative voltage responses of first spikes elicited from FSIs at rheobase (Ith); Ith+100 pA; Ith+200; and Ith+300 pA current steps. For clarity, only the initial spike in each trace is displayed, and the intervening, +50 pA, current steps are omitted. (C, D) Phase plots of the traces shown in A, B. Phase plots are shown for the first spike discharged during injection at Ith and Ith +: 50, 100, 150, 200, 250 and 300 pA. (E, F) Regions of interest indicated by the dashed line are displayed at higher magnification. Red dashed line indicates spike threshold (dV/dt = 10). Insets are plots of injected current amplitude versus first spike threshold of displayed cell. (G, H) Plots of injected current amplitude versus first spike threshold. (I) Mean slope of injected current amplitude versus first spike threshold in (G, H). Values are means ± S.E.M. **P<0.005, vs. control by ANOVA.