Lymphocyte Activation Gene 3 (LAG-3) Modulates the Ability of CD4 T-cells to Be Suppressed In Vivo
Figure 5
Decreased FOXP3 Treg induction in LAG-3 KO cells in vitro.
WT or LAG-3 KO 6.5 TCR Transgenic CD4+ T-cells skewed in Th1 or Treg Conditions and analyzed for A) FOXP3 or B) TBET Expression. C) 6.5 TCR transgenic CD4+ T-cells were isolated and mixed with matched splenocytes 1∶3 and stimulated with 1 or 10 µM HA peptide for 3 days. WT or LAG-3 KO 6.5 CD4+ T-cells were treated with αLAG-3 or isotype control antibody at 50 µg/mL and cells were stained for P-STAT5. D) Summary graph of two experiments. Data shown are representative of at least two independent experiments.