Ras GTPase Activating Protein CoIra1 Is Involved in Infection-Related Morphogenesis by Regulating cAMP and MAPK Signaling Pathways through CoRas2 in Colletotrichum orbiculare
Figure 14
Assembly of F-actin in the appressorium pores of the coira1 mutant.
(A) Micrograph of F-actin organization in the appressorium pore visualized by the expression of Lifeact–RFP in the wild-type and in the coira1 mutant. Conidial suspensions (10 µl) of each strain were applied to the abaxial surface of the cucumber cotyledons and incubated at 24°C for 48 h. WT/RA, the wild type expressing Lifeact–RFP; iRA, the coira1 mutant expressing Lifeact–RFP (B) Percentage of the assembly of F-actin in the appressorium pore of the coira1 mutants on the abaxial surface of cucumber cotyledons. Approximately 100 appressoria of each strain were observed per incubated site for 48 h, 72 h post-inoculation. Two replicates were examined. Three independent experiments were conducted, and standard errors are shown. Black bar, WT/RA; gray bar, Ira; WT/RA, the wild type expressing LifeAct-RFP; iRA, the coira1 mutant expressing LifeAct-RFP.