Ras GTPase Activating Protein CoIra1 Is Involved in Infection-Related Morphogenesis by Regulating cAMP and MAPK Signaling Pathways through CoRas2 in Colletotrichum orbiculare
Figure 5
Intracellular cAMP levels in the coira1 mutant.
Intracellular cAMP levels were measured in tissue collection from three-day old liquid cultures of each strain. WT, the wild-type 104-T; DL1-1, the coira1 mutant; CL1-1, the CoIRA1-complemented transformant of DL1-1; DARS1, WT transformed with a dominant active form CoRAS1; DARS2, WT transformed with a dominant active form CoRAS2; iDNRS1, DI1-1 transformed with a dominant negative form CoRAS1; iDNRS2, DI1-1 transformed with a dominant negative form CoRAS2; DC1, the cac1 mutant. Three independent experiments were conducted, and standard errors are shown.