Podocalyxin Regulates Murine Lung Vascular Permeability by Altering Endothelial Cell Adhesion
Figure 2
Cdh5-Cre drives efficient deletion of podocalyxin in the lung.
(A) Podocalyxin expression is completely abrogated in the lung of PodxlΔEC mice. (B, C) Within the aorta and vessels of the small intestine (arrows), podocalyxin is deleted in all but a few isolated cells in PodxlΔEC mice. (D) Within the kidney, podocalyxin (brown staining) is efficiently deleted in the glomerular endothelial cells (arrows, 40x mag. panels) and larger vessels (arrowheads, 10x mag. panels). Positive staining in the glomerulus of PodxlΔEC kidney are likely podocyte epithelial cells. Adjacent H&E sections included demonstrating normal morphology with loss of podocalyxin expression in these tissues.