Podocalyxin Regulates Murine Lung Vascular Permeability by Altering Endothelial Cell Adhesion
Figure 1
Conditional deletion of the Podxl locus in endothelial cells.
(A) Schematic representation of the Podxl transgenic allele, floxed allele (PodxlF/F) and deleted allele (PodxlΔF/F). Exons are depicted as vertical lines. Inserted loxP and frt sites are depicted with black and grey arrowheads, respectively. The NeoR cassette is represented by a box. (B) Capillary gel electrophoresis of genomic DNA isolated and amplified (PCR) from primary lung endothelial cells prepared from mice harboring wild type (WT, 122 bp), floxed (Flox, 171 bp) or functionally deleted (via Cdh5-Cre) Podxl alleles (ΔFlox, 285 bp) (C) qRT-PCR evaluation of podocalyxin mRNA in highly vascularized adult tissues harvested from PodxlF/F (black bars) and PodxlΔEC (white bars) mice (n = 3–6). Expression levels were quantified relative to Gapdh and then normalized to the mean Podxl expression in the PodxlF/F tissues. *Significantly different compared to PodxlF/F mouse tissue where P<0.05 by Student's t test. Error bars represent the SEM.