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Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

Figure 2

DGGE analyses of mglA gene fragments performed using DNA samples extracted from pure myxobacteria cultures (A) and soil samples (B).

A lane, 1, Archangium gephyra DSM 2261; lane 2, Corallococcus macrospores DSM 14697; lane 3, Corallococcus coralloides DSM 52499; lane 4, Myxococcus fulvus DSM 16525; lane 5, Myxococcus virescens DSM 2260; lane 6, Cystobacter minus DSM 14751; lane 7, Stigmatella erecta DSM 16858; lane 8, Byssovorax cruenta DSM 14533; B, Bands (1∼28) that were excised for sequence analysis are numbered.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0108877.g002