Thy1-GCaMP6 Transgenic Mice for Neuronal Population Imaging In Vivo
Figure 5
ALM functional imaging using GP4.3 during behavior.
a. Schematic of the object localization behavior (see ref. [34] for details). b. An image of GCaMP6s labeled neurons in a GP4.3 mouse, 160 µm below the pia. Blue and red colors indicate cells that responded during lick-right and lick-left trials, respectively. Cue = auditory “go” signal. c. Fluorescent responses of three example cells indicated in b. d. Top, single-trial responses of the same neurons in b sorted according to trial type (blue: lick-right, red: lick-left). Bottom, trial-averaged response. e. The responses of cell 3 measured over 4 behavioral sessions spanning more than one month. f. Peak normalized fluorescent transients of GCaMP expressing neurons in GP5.17, GP4.3 and AAV-GCaMP6s injected mice (0.2±0.1 s, 0.65±0.35 s, and 1.8±1.1s mean±s.d; n = 207, 771, and 369 cells for GP5.17, GP4.3, and AAV-6s respectively).