Down-Regulation of Desmosomes in Cultured Cells: The Roles of PKC, Microtubules and Lysosomal/Proteasomal Degradation
Figure 5
Desmosomal proteins remain co-localised following internalisation.
(A–D) Co-localisation of the desmosomal proteins DP, PG, Dsc2a and Dsg2 in NCM persists following LCM-induced internalisation for 1 hour (A,B) and 24 hours (C,D). Yellow arrows indicate XZ axis. Bar, 5 µm. Fluorescence profiles depict the intensity of staining along the white line in the merged images. (E) Cells were treated with either NCM or LCM for 90 minutes and then separated into their insoluble (INS) and soluble (SOL) fractions. Western blots for desmosomal proteins (E) were quantified by densitometry (F) (dashed lines indicate lanes which have been re-ordered from the same western blot). Asterisk indicates statistical significance (p<0.0286, Mann-Whitney test). For further details see text.