Sustained Induction of Collagen Synthesis by TGF-β Requires Regulated Intramembrane Proteolysis of CREB3L1
Figure 5
TGF-β induces cleavage of CREB3L1 through activation of ERKs.
(A) A549 cells were set up, treated, and analyzed with immunoblot with indicated antibodies as described in Fig. 1C. (B–D) On day 0, A549 cells were seeded at 4×105 cells per 60 mm dish. On day 1, cells were treated with 0.5 µM RDEA119 or PD0325901 for 3 h followed by treatment with 1 ng/ml TGF-β as indicated. On day 2, 24 h after the TGF-β treatment, cells were harvested for immunoblot analysis with indicated antibodies (B and D) and quantification of TM4SF20 mRNA with RT-QPCR, with the amount of the mRNA in untreated cells set to 1 (Results are reported as mean ± S.E.M. of three independent experiments) (C).